2007) would be consistent with a conserved role for SBDS in 60S subunit maturation, the current model in mammalian cells posits that e IF6 removal is triggered following phosphorylation of Ser 235 by protein kinase C (PKC) and RACK1 (receptor for activated protein C) (Ceci et al. Furthermore, diverse alternate functions for SBDS in mammalian cells have been suggested, including mitotic spindle stabilization (Austin et al. 2006), Fas ligand-induced apoptosis (Rujkijyanont et al. 2009), and Rac2-mediated monocyte migration (Leung et al. Thus, despite the prior genetic studies in yeast, the mechanism of e IF6 release in mammalian cells is controversial, biochemical evidence supporting direct catalysis of e IF6 release by SBDS and EFL1 in eukaryotic cells is currently lacking, and the specific function of the SBDS protein, its mode of action, and the molecular mechanism of the cooperative interaction with EFL1 remain obscure.
To resolve these issues, we solved a high-resolution NMR structure of the human SBDS protein and biochemically reconstituted e IF6 release for the first time ex vivo using genetically stalled pre-60S subunits isolated from -deleted mice.
Furthermore, complementary NMR studies suggest unanticipated mechanistic parallels between this late step in 60S ribosome biogenesis and aspects of bacterial ribosome disassembly.
By elucidating the conserved mechanism of e IF6 release in eukaryotes, this study provides compelling evidence that SDS is a ribosomopathy caused by uncoupling GTP hydrolysis by EFL1 from e IF6 release on the ribosome.
To test this, liver cells were fractionated into cytoplasmic, soluble, and insoluble nuclear extracts and immunoblotted to detect e IF6. Furthermore, Ebp1, the mammalian ortholog of the yeast 60S biogenesis factor Arx1, did not appear to accumulate or show redistribution upon , we hypothesized that the cytoplasmic accumulation of late pre-60S assembly factors was most likely due to their retention on late pre-60S particles.