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This may occur when an Rh-positive baby is born to an Rh-negative mother.

Formerly, antibodies to the Rh antigen were the most common cause of hemolytic disease of the newborn, but this condition is now rare due to preventive treatments given to the mother during and after each pregnancy.

If the antibody is found coating the RBCs, then the RBCs may be destroyed (hemolyzed) or be removed from circulation faster than normal. In general, the stronger the DAT reaction (the more positive the test), the greater the amount of antibody bound to the RBCs, but this does not always equate to the severity of symptoms, especially if the RBCs have already been destroyed.