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Carbohydrates were the most abundant macronutrients (57.83 g/100 g DM) followed by proteins (28.81 g/100 g DM) in T. The ash content was 5.06 g/100 g DM, and potassium, calcium, phosphorus, magnesium and iron were found to be particularly abundant.The results of ascorbic acid, total carotenoids and total anthocyanins expressed on a dry mass truffle were 10.63 mg/100 g, 1.17 mg/100 g and 29.1 mg/100 g, respectively. nivea contained relatively important amounts of total phenolics and flavonoids.

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Then, the macerate was filtered through Whattman No.1 filter paper.

The same procedure was repeated twice with the obtained residue, and then the total filtrate (macerate) was lyophilized.

Chemicals DPPH• and chemical standards were purchased from Sigma Aldrich Co. Truffles fruiting bodies were collected during the months of March and April 2013 from southern area of Tunisia (Medenine, Benguerdenne: latitude 32°57’09’’N, longitude 11°38’26’’E, with arid climate characterized by a mean rainfall of 150 mm/year).